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ID 32031
file
creator
Sakai, Noriyuki
Nagahara, Takayoshi
Kawaguchi, Hiroyuki
NDC
Medical sciences
abstract
Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.
journal title
Tissue engineering. Part A.
volume
Volume 17
issue
Issue 7-8
start page
955
end page
967
date of issued
2011
publisher
Mary Ann Liebert
issn
1937-3341
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
This is a copy of an article published in the Tissue engineering, Part A. (c) 2011 Mary Ann Liebert, Inc.; Tissue engineering, Part A. is available online at: http://online.liebertpub.com.
relation is version of URL
http://dx.doi.org/10.1089/ten.TEA.2010.0070
department
Graduate School of Biomedical Science