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ID 47055
file
creator
Tamura, Takayoshi
Ozaki, Moeko
Maruyama, Masafumi
subject
plant-derived lactic acid bacteria
Enterococcus avium
gamma-aminobutyric acid
NDC
Medical sciences
abstract
In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7±6.4 g/l GABA at a conversion rate of 86.0±5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG.
description
This work was supported by Hiroshima Biocluster (a Cooperative Link of Unique Science and Technology for Economy Revitalization), Japan (M.S.).
journal title
Biological and Pharmaceutical Bulletin
volume
Volume 33
issue
Issue 10
start page
1673
end page
1679
date of issued
2010-10-01
publisher
The Pharmaceutical Society of Japan
issn
0918-6158
1347-5215
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
© 2010 Pharmaceutical Society of Japan
relation url
department
Graduate School of Biomedical & Health Sciences