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ID 17091
file
creator
Akimoto, Yukimi
Suzuki, Tomoharu
Kitamura, Shigeyuki
subject
mouse prostate
secretion
proteome analysis
lobe specific
androgen regulation
NDC
Medical sciences
abstract
Rats and guinea pigs have frequently been used to study the development of the prostate and the mechanism of androgen action, but the mouse prostate has also become an attractive model for prostate research, because an enormous range of genetically altered mice is now available. However, the secretion of proteins in the mouse prostate has not yet been well investigated. In the present study, major secreted proteins from the ventral (VP), dorso-lateral (DLP) and anterior prostate (AP) of mice were identified by means of two-dimensional gel electrophoresis followed by MALDI-TOF mass spectrometric analysis. A quantitative RT-PCR method was further employed to examine the androgen-dependent transcriptional regulation of the identified proteins. Proteome analysis revealed that the VP secretes spermine binding protein, serine protease inhibitor Kazal type 3 and a 91 kDa hypothetical scavenger receptor (AK035662). The DLP and the AP secrete a protein similar to immunoglobulin binding protein (IgBPLP) and one of the experimental autoimmune prostatitis antigen proteins (EAPA2). Peroxiredoxin-6, glucose-regulated protein 78, zinc-a2-glycoprotein and phospholipase Ca are also secreted. Castration of animals led to a decrease in the mRNAs of these secreted proteins, although the extents of change varied greatly among different lobes. We present here an overview of mouse prostate secretion, which should contribute to an understanding of the biological functions of the prostate gland, as well as the androgen-dependency of prostate secretion.
journal title
Journal of Endocrinology
volume
Volume 190
issue
Issue 3
start page
793
end page
803
date of issued
2006-09
publisher
Elsevier
issn
0022-0795
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd.
relation is version of URL
http://dx.doi.org/10.1677/joe.1.06733
department
Research Institute for Radiation Biology and Medicine