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ID 14745
file
creator
Yuge, Osafumi
Ninomiya, Yuichi
subject
22(R)-Hydroxycholesterol
Inflammation
Macrophage
22R-HC
NDC
Medical sciences
abstract
Liver X receptors (LXRs) play an important role in lipid metabolism. Recently, a role for these proteins was identified in suppressing the inflammatory response. However, it is not known whether the natural ligands of LXRs, e.g. 22(R)-hydroxycholesterol (22R-HC), can suppress the inflammatory response after the onset of inflammation. We demonstrate here that treatment of Lipopolysaccharide (LPS)-activated RAW264.7 macrophages with 22R-HC markedly suppressed nitric oxide (NO) production and inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression. Additionally, 22R-HC did not affect the DNA binding activity of NF-κB, AP-1 and C/EBP(s), important transcriptional factors for iNOS and COX-2 genes expression. Furthermore iNOS and COX-2 mRNA suppression by 22R-HC was diminished by cellular treatment with cycloheximide. These results suggest that 22R-HC suppresses the expression of iNOS and COX-2 genes through de novo protein synthesis of an unidentified protein in LPS-activated macrophages. © 2005 Elsevier Ltd. All rights reserved.
journal title
The Journal of Steroid Biochemistry and Molecular Biology
volume
Volume 97
issue
Issue 4
start page
376
end page
383
date of issued
2005-12
publisher
Elsevier Ltd
issn
0960-0760
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2005 Elsevier Ltd
relation is version of URL
http://dx.doi.org/10.1016/j.jsbmb.2005.06.030
department
Graduate School of Biomedical Science