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ID 15040
file
creator
Onimatsu, Hideki
Suganuma, Kei
Uenoyama, Shushi
subject
C-terminal repetitive motif
Cell wall binding protein
Chlorovirus
Unique vertex
Vp130
NDC
Botany
abstract
Previously, Vp130, a chloroviral structural protein, was found to have host-cell-wall-binding activity for NC64A-viruses (PBCV-1 and CVK2). In this study, we have isolated and characterized the corresponding protein from chlorovirus CVGW1, one of Pbi-viruses that have a different host range. In NC64A-viruses, Vp130 consists of a highly conserved N-terminal domain, internal repeats of 70-73 aa motifs and a C-terminal domain occupied by 23-26 tandem repeats of a PAPK motif. However, CVGW1 was found to have a slightly different Vp130 construction where the PAPK repeats were not in the C-terminus but internal. Immunofluorescence microscopy with a specific antibody revealed that the C-terminal region containing the Vp130 repetitive motifs from PBCV-1 and CVK2 was responsible for binding to Chlorella cell walls. Furthermore, by immunoelectron microscopy and immunofluorescence microscopy, Vp130 was localized at a unique vertex of the chlorovirus particle and was found to be masked through binding to the host cells. These results suggested that Vp130 is localized at a unique vertex on the virion, with the C-terminal repetitive units outside for cell wall binding.
journal title
Virology
volume
Volume 353
issue
Issue 2
start page
433
end page
442
date of issued
2006-09
publisher
Elsevier Inc.
issn
0042-6822
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Inc.
relation is version of URL
http://dx.doi.org/10.1016/j.virol.2006.06.010
department
Graduate School of Advanced Sciences of Matter