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ID 34823
file
creator
Takatsu, Satomi
Ishiwata, Takayuki
Tanaka, Hideto
Sarre, Sophie
Meeusen, Romain
subject
Microdialysis
Exercise
Thermoregulation
Neurotransmission
NDC
Biology
abstract
The purpose of the present study was to investigate the relationship between thermoregulation and catecholamine release in the preoptic area and anterior hypothalamus (PO/AH) during incremental treadmill running in the rat. To this aim, we combined in vivo brain microdialysis, biotelemetry and metabolic measurements for continuous monitoring of core body temperature (T(core)), neurotransmitters and thermoregulatory responses. The animals were exercised for 1 h at 23 degrees C. Treadmill speed was increased every 20 min (10, 20 and 26 m min(-1)). T(core). oxygen consumption (VO(2), an index of heat production) and tail skin temperature (T(tail), an index of heat loss) were simultaneously measured. Brain microdialysis samples were collected every 10 min, and these samples were analyzed for noradrenaline (NA), dopamine (DA) and serotonin (5-HT). T(core) and VO(2) significantly increased during treadmill and were exercise intensity dependent. After an initial drop T(tail) increased significantly during exercise. Both NA and DA levels in the PO/AH increased significantly during exercise. There was no effect on serotonin release. T(core). VO(2) and T(tail) were positively correlated with the levels of NA and DA. Our data suggest that thermoregulatory responses are dependent on the intensity of the exercise and that these responses are associated with changes in NA and DA release, but not in 5-HT release in the PO/AH.
journal title
Journal of Neuroscience Methods
volume
Volume 202
issue
Issue 2
start page
119
end page
123
date of issued
2011
publisher
Elsevier Science BV
issn
0165-0270
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
(c) 2011 Elsevier B.V. All rights reserved.
relation url