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ID 28691
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title alternative
Phage Therapy against β-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus
creator
Matsuoka, Satoru
Hashizume, Takaya
Kanzaki, Hiroyuki
Iwamoto, Emi
Park, Se Chang
Yoshida, Terutoyo
subject
bacteriophages
phage therapy
β-hemolytic streptococcicosis
Streptococcus iniae
Paralichthys olivaceus
Japanese flounder
NDC
Zoology
abstract
We examined the therapeutic effect of Streptococcus iniae phages isolated from fish culture environments against experimental streptococcicosis of Japanese flounder Paralichthys olivaceus. Phage sensitivity tests with a double agar method revealed that 31 of 35 S. iniae strains from the flounder have a similar sensitivity to six phage isolates. In phage therapy experiments, fish were injected intraperitoneally (IP) with S. iniae PSi402 and 1 h later IP-injected with a mixture of two or four phage isolates, and observed at 25°C for 2 wk. Mortalities of fish receiving phages were significantly lower than those of control fish without phage-treatment in all four trials. The effect of phage treatment was also demonstrated even at 24 h post-infection, when cell numbers of S. iniae were 10^7.4 and 10^4.5 CFU/g in the kidneys and brains of fish, respectively. However, as phage-resistant S. iniae were frequently isolated from dead fish in the phage-treated group, further investigations are required to establish phage therapy of the disease.
journal title
魚病研究
volume
Volume 42
issue
Issue 4
start page
181
end page
189
date of issued
2007-12-15
publisher
日本魚病学会
issn
0388-788X
1881-7335
ncid
publisher doi
language
jpn
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2007 The Japanese Society of Fish Pathology
relation url
department
Graduate School of Biosphere Science