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ID 19835
file
creator
Koyama, Hirofumi
Itoh, Masayuki
Miyahara, Kohji
subject
RSC
nucleosome-remodeling complex
NPS1/STH1
Saccharomyces cerevisiae
bromodomain
DNA damage
NDC
Biology
abstract
The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome-remodeling complex, RSC, of Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature-sensitive mutant allele of NPS1, nps1-13, which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA-damaging treatments at the permissive temperature. Reduced expression of NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage
journal title
FEBS Letters
volume
Volume 531
issue
Issue 2
start page
215
end page
221
date of issued
2002-11-06
publisher
Elsevier
issn
0014-5793
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2002 Federation of European Biochemical Societies
relation is version of URL
http://dx.doi.org/10.1016/S0014-5793(02)03504-4
department
Graduate School of Advanced Sciences of Matter