このエントリーをはてなブックマークに追加
ID 34541
file
creator
Awata, Takanori
Suzuki, Yuji
Tanabe, Katsuichiro
Hatamoto, Masashi
Ohashi, Akiyoshi
subject
anaerobic ammonium oxidation (anammox)
marine anammox bacteria
“Candidatus Scalindua" species
enrichment culture
FISH
phylogenetic analysis
NDC
Pollution. Environmental engineering
abstract
We established an enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria using an up-flow column reactor fed with artificial sea water supplemented with nitrogen and minerals and inoculated with coastal surface sediment collected from Hiroshima Bay. After 2 months of reactor operation, simultaneous removal of NH4+ and NO2- was observed, suggesting that an anammox reaction was proceeding. A total nitrogen removal rate of 2.17 g-N L-1 day-1 was attained on day 594 while the nitrogen loading rate was 3.33 g-N L-1 day-1. Phylogenetic analysis revealed that at least two dominant “Candidatus Scalindua" species were present in this reactor. Moreover, many uncultured bacteria and archaea, including candidate division or ammonia-oxidizing archaea, were present. Fluorescence in situ hybridization (FISH) revealed that anammox bacteria accounted for 85.5 ± 4.5% of the total bacteria at day 393. We also designed two oligonucleotide probes specific to each dominant “Candidatus Scalindua" species. A simultaneous FISH analysis using both probes showed that two different “Candidatus Scalindua" species were clearly recognizable and coexisted during reactor operation, although there was some variation in their abundance. The marine anammox bacteria enriched in this study have potential applications to the treatment of industrial wastewater containing high levels of ammonium and salt.
journal title
Microbes and Environments
volume
Volume 26
issue
Issue 1
start page
67
end page
73
date of issued
2011
publisher
日本微生物生態学会
日本土壌微生物学会
issn
1342-6311
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM)
relation url
department
Graduate School of Engineering