Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains
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For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.
This work was supported in part by the Japanese Ministry of Education, Science, Sports, and Culture, Grant-in-Aid for Scientific Research (grant 20570221).
Journal of Microbiological Methods
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© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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Graduate School of Science
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