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ID 46881
file
creator
subject
Agrobacterium
plasmid removal
Ti plasmid
Toxin-antitoxin system
NDC
Zoology
abstract
For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.
description
This work was supported in part by the Japanese Ministry of Education, Science, Sports, and Culture, Grant-in-Aid for Scientific Research (grant 20570221).
journal title
Journal of Microbiological Methods
volume
Volume 89
issue
Issue 1
start page
53
end page
56
date of issued
2012-04
publisher
Elsevier B.V.
issn
0167-7012
1872-8359
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
relation url
department
Graduate School of Science



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