このエントリーをはてなブックマークに追加
ID 46813
file
creator
Kataoka, Naoya
Rachadech, Wanitcha
Alisa, S Vangnai
subject
Organic-solvent tolerant bacteria
Butanol-tolerant bacteria
Heterologous gene-expression host
abstract
As alternative microbial hosts for butanol production with organic-solvent tolerant trait are in high demands, a butanol-tolerant bacterium, Bacillus subtilis GRSW2-B1, was thus isolated. Its tolerance covered a range of organic solvents at high concentration (5%v/v), with remarkable tolerance in particular to butanol and alcohol groups. It was susceptible for butanol acclimatization, which resulted in significant tolerance improvement. It has versatility for application in a variety of fermentation process because it has superior tolerance when cells were exposed to butanol either as high-density, late-exponential grown cells (up to 5%v/v) or under growing conditions (up to 2.25%v/v). Genetic transformation procedure was optimized, yielding the highest efficiency at 5.17 × 103 colony forming unit (μg DNA)-1. Gene expression could be effectively driven by several promoters with different levels, where as the highest expression was observed with a xylose promoter. The constructed vector was stably maintained in the transformants, in the presence or absence of butanol stress. Adverse effect of efflux-mediated tetracycline resistance determinant (TetL) to bacterial organic-solvent tolerance property was unexpectedly observed and thus discussed. Overall results indicate that B. subtilis GRSW2-B1 has potential to be engineered and further established as a genetic host for bioproduction of butanol.
description
This work was the collaboration of Chulalongkorn University - Hiroshima University under the Asian Core Program (ACP) and financially supported by The Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT) (Bilateral Project). It was partly supported by the Thai Government Stimulus Package 2 (TKK2555) under the Project for Establishment of Comprehensive Center for Innovative Food, Health Products and Agriculture (PERFECTA).
journal title
AMB Express
volume
Volume 1
issue
Issue 1
start page
10
date of issued
2011-05-30
publisher
Springer Nature
issn
2191-0855
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
© 2011 Kataoka et al; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
relation url
department
Graduate School of Advanced Sciences of Matter



Last 12 months's access : ? times
Last 12 months's DL: ? times


This month's access: ? times
This month's DL: ? times