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ID 38273
file
title alternative
ヒト乳癌細胞株(YMB-1)の樹立とその特性
creator
Yamane, Motoi
Nishiki, Masayuki
Kishi, Naohiko
Amano, Kuniki
Nakagawa, Kenichi
Okumichi, Tsuneo
Naito, Masashi
Ito, Akihiko
Ezaki, Haruo
subject
Established cell line
Human breast cancer
NDC
Medical sciences
abstract
A continuous cell line (YMB-1), derived from a primary breast cancer lesion has been established and maintained in culture for the past 9 months. Cell colonies consist of polygonal epithelial cells containing junctional complexes and tubular formation. The cell population doubling time is 44 hr and the saturation density at confluency is 2.5×10^5 cells/cm2. The chromosome modal number is 73 and 3 marker chromosomes are visible. Both cytosol estrogen and androgen receptors are present. However, the cytosol progesterone receptor was not detected by the dextran coated chacoal method. The s. c. injection of 5×10^6 cells from cell culuture into nude mice induced a tumor.

Both the spillage method and cell separation by specific gravity were employed to remove fibroblasts.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 33
issue
Issue 4
start page
715
end page
720
date of issued
1984-12
publisher
Hiroshima University School of Medicine
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
Research Institute for Radiation Biology and Medicine
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