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ID 27700
file
creator
Urata, Masaaki
Iwata, Rei
subject
ATP assay
Immunochromatographic lateral flow assay
Mutant firefly luciferase
Salmonella enteritidis
NDC
Chemical technology
abstract
ATP-based bioluminescence using mutant firefly luciferase was combined with an immunochromatographic lateral flow test strip assay for Salmonella enteritidis detection. In this combination method, the Salmonella-antibody-gold complex captured at the test line on the test strip was lysed by heat-treatment, and the ATP released from the cells was measured using mutant luciferase. This method resulted in approximately 1,000 times higher sensitivity in the detection of Salmonella (i.e. 10(3) c.f.u./ml) compared to immunochromatographic lateral flow assay.
journal title
Biotechnology Letters
volume
Volume 31
issue
Issue 5
start page
737
end page
741
date of issued
2009
publisher
Springer
issn
0141-5492
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2009 Springer-Verlag. "The original publication is available at www.springerlink.com"
relation url
department
Research Center for Nanodevices and Systems



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