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ID 26302
file
creator
Sakamoto, Kiyako
Noguchi, Kazuyuki
Somerman, Martha J.
subject
prostaglandin E2
PGE receptor subtypes
cementoblasts
periodontal tissue
NDC
Medical sciences
abstract
Objective: PGE2 is an important factor in pathogenesis of periodontal disease because of bone resorting activity and association with attachment loss. PGE2 and PGE receptor subtypes (EPs) play an important role in modulating bone metabolism via osteoblasts. However, little is known about the effects of PGE2 on cementoblasts. The aims of this study were to determine the expression of EPs on matured cementoblasts and to examine the effect of PGE2 and EPs on their cellar function. Design: Expression of EPs in immortalized mouse cementoblasts (OCCM-30), which were characterized as matured cementoblasts, was determined using RT-PCR. Then effects of PGE2 and EP agonists on mineralization were examined by studying nodule formation with alizarin red S (ALZ) staining. Alkaline phosphatase (ALP) activity with PGE2, EP4 agonist was examined by Bessey-Lowry enzymologic method. Effects of PGE2-EP4 pathway on expression levels of osteocalcin (OCN) and matrix metalloproteinase (MMP)-13 mRNA were examined by real-time RT-PCR. Results: OCCM-30 expressed EP1, 2, 3 and 4 mRNA. PGE2 and EP4 agonist caused downregulation of mineralized nodule formation and ALP activity in OCCM-30. OCN mRNA expression was suppressed and MMP-13 mRNA expression was stimulated via PGE2-EP4 pathway in OCCM-30. Conclusions: Cementoblasts may downregulate their mineralization ability and upregulate MMP-13 production through PGE2-EP4 pathway and may contribute to destruction of connective tissue attachment under inflammatory condition.
journal title
Archives of Oral Biology
volume
Volume 53
issue
Issue 3
start page
243
end page
249
date of issued
2008
publisher
Elsevier Ltd
issn
0003-9969
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2007 Elsevier Ltd
relation is version of URL
http://dx.doi.org/10.1016/j.archoralbio.2007.10.007
department
Graduate School of Biomedical Science



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