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ID 25995
file
title alternative
EFFECTS OF HINDLINB SUSPENSION ON PATTERNS OF MYOSIN ISOFORMS IN RAT SOLEUS MUSCLE
creator
Okumoto, Tadashi
Saitoh, Ayako
Katsuta, Shigeru
Niihata, Shigemitsu
Kikuchi, Kunio
subject
hindlimb suspension
isomyosins
myosin heavy chain isoforms
myosin light chain isoforms
NDC
Medical sciences
abstract
Using several electrophoretic techniques, this study examined the efeicts of 3 weeks hindlimb suspension on the patterns of isomyosins, myosin heavy chain(HC)isoforms and myosin light chain (LC)isoforms in the soleus muscle of the rat. The suspended soleus showed a shift in the HC isoform distribution with a marked increase in fast HC isoforms and a commensurate decrease in HCI. In addition, the change in the fast HC isoforms consisted of the expression of HCIId and HC IIb absent in the normal soleus. In contrast to HC isoforms, suspension did not lead to appreciable changes in LC isoform distribution. Analyses of electrophoresis under nondenaturing conditions demonstrated that the normal soleus expressing HCI and HCIIa isoforms contained two isomysoins. Although, of the two isomyosins observed in the normal soleus, the faster migrating band most likely represented the HCIIa-based one(FMas), its mobility was not identical with that of the HCIIa-based isomyosin(FMaf)found in fast-twitch muscles, migrating in the order FMaf>FMas. FMas was designated as intermediate isomyosin(IM). Some of the suspended soleus contained slow isomyosin(SM)and IM whereas the others comprised FM3 and/or FM2 as well as SM and IM. In spite of the expression of HCIIb and HCIId in the suspended soleus, FM3 and FM2 observed in these muscles exhibited distinct mobilities from either HCIId-based or HCIIb-based isomyosins comprised in fast-twitch muscles. These results suggest that some of newly expressed HCIId and /or HCIIb isoforms in the suspoended soleus are associated with not only fast but also slow LC isoforms and function as a constitutive elemet of the myosin molecule.
journal title
体力科学 : Japanese Journal of Physical Fitness and Sports Medicine
volume
Volume 46
issue
Issue 3
start page
279
end page
287
date of issued
1997-06-01
publisher
日本体力医学会
issn
0039-906X
ncid
language
jpn
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
日本体力医学会
本文データは学協会の許諾に基づきCiNiiから複製したものである
relation url
department
Graduate School of Integrated Arts and Sciences



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