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ID 14339
file
creator
Terada, Takato
NDC
Zoology
abstract
The objective of this study was to examine the effect of bovine follicular fluid added to the matura-tion medium on sperm penetration, male pronucleus formation and polyspermy in pig oocytes fertilizedin vitro. Pig oocytes were cultured in 10 µl droplets of medium without bovine follicular fluid for 24 h.The oocytes were then cultured in 100 µl droplets of medium supplemented with 0,10 or 20% bovinefollicular fluid for further 24 h. The cultured oocytes were inseminated with boar sperm at a concentra-tion of 1×105or 106sperm/ml. When oocytes were inseminated at 1×105sperm/ml, significantly lowerproportion of penetrated oocytes was found in 20% bovine follicular fluid group compared with that in 0and 10% bovine follicular fluid groups (P<0.05). Addition of bovine follicular fluid to maturation medi-um promoted the male pronucleus formation in oocytes inseminated with 1×105sperm/ml, with a sig-nificant (P<0.05) difference in rate of oocytes with male pronuclei between 0 0x1.de8c8p-891nd 10% bovine follicu-lar fluid groups. Treatment with bovine follicular fluid during the second 24 h culture significantlydecreased the proportions of polyspermic oocytes inseminated with either of sperm concentrations.These results indicate that the presence of bovine follicular fluid in maturation medium enhances malepronucleaus formation and reduces polyspermic penetration in pig oocytes fertilized in vitro.
journal title
Journal of International Development and Cooperation
volume
Volume 7
issue
Issue 1
start page
59
end page
66
date of issued
2001-01
publisher
広島大学国際協力研究科
issn
1341-0903
ncid
SelfDOI
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School for International Development and Cooperation
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