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ID 25
file
creator
Uyama, Taro
Kanamori, Kan
Michibata, Hitoshi
subject
ascidian
metal accumulation
vacuolar H+-ATPase
vacuolre
gene expression
NDC
Biology
abstract
A vanadium-accumulating ascidian, Ascidia sydneiensis samea, expresses vacuolar-type H+-ATPases (V ATPases) on the vacuole membrane of the vanadium-containing blood cells known as vanadocytes. Previously, we showed that the contents of their vacuoles are extremely acidic and that a V ATPase-specific inhibitor, bafilomycin A1, neutralized the contents of the vacuoles. To understand the function of V ATPase in vanadocytes, we isolated cDNA encoding subunit C of V ATPase from vanadocytes since this subunit has been known to be responsible for the assembly of V-ATPases and to regulate the ATPase activity of V-ATPases. The cloned cDNA was 1,443 nucleotides in length, and encoded a putative 384 amino-acid protein. By expressing the ascidian cDNA for subunit C under the control of a galactose-inducible promoter, the pH-sensitive phenotype of the corresponding vma5 mutant of a budding yeast was rescued. This result showed that the ascidian cDNA for subunit C functioned in yeast cells.
journal title
Marine biotechnology
volume
Volume 3
start page
316
end page
321
date of issued
2001
publisher
Springer
issn
1436-2228
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2001 Springer "The original publication is available at www.springerlink.com"
relation is version of URL
http://dx.doi.org/10.1007/s1012601-0054-x
department
Graduate School of Science



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