A Simple Method for the Observation of Cultured Rat Hepatocytes by Scanning Electron Microscopy

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タイトル ( eng )
A Simple Method for the Observation of Cultured Rat Hepatocytes by Scanning Electron Microscopy
作成者
KASUGA Shigeo
TAGASHIRA Nobuharu
HARADA Yasuo
収録物名
Hiroshima Journal of Medical Sciences
36
2
開始ページ 169
終了ページ 174
収録物識別子
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
抄録
A simple method for the three-dimensional observation of inner and outer ultrastructures of the cultured hepatocyte was presented. The freeze cracked plane of the cultured cell was obtained by means of cracking the underlying substrate. This method made it possible to observe the ultrastructural features of both cell surface and cytoplasm in the same cell. Among several substrates examined in this investigation, the collagen gel sheet was the most useful for this purpose.
著者キーワード
Cultured hepatocytes
Scanning electron microscopy
Three-dimensional structure
Aldehyde-prefix osmium-DMSO-osmium method
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 紀要論文
出版者
Hiroshima Journal Medical Press
発行日 1987-06
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3654268