The conserved Wdr8-hMsd1/SSX2IP complex localises to the centrosome and ensures proper spindle length and orientation

Hori Akiko

Morand Agathe

Ikebe Chiho

Frith David

Snijders Ambrosius P.

Toda Takashi

Biochemical and Biophysical Research Communications

The centrosome plays a pivotal role in a wide range of cellular processes and its dysfunction is causally linked to many human diseases including cancer and developmental and neurological disorders. This organelle contains more than one hundred components, and yet many of them remain uncharacterised. Here we identified a novel centrosome protein Wdr8, based upon the structural conservation of the fission yeast counterpart. We showed that Wdr8 constitutively localises to the centrosome and super resolution microscopy uncovered that this protein is enriched at the proximal end of the mother centriole. Furthermore, we identified hMsd1/SSX2IP, a conserved spindle anchoring protein, as one of Wdr8 interactors by mass spectrometry. Wdr8 formed a complex and partially colocalised with hMsd1/SSX2IP. Intriguingly, knockdown of Wdr8 or hMsd1/SSX2IP displayed very similar mitotic defects, in which spindle microtubules became shortened and misoriented. Indeed, Wdr8 depletion resulted in the reduced recruitment of hMsd1/SSX2IP to the mitotic centrosome, though the converse is not true. Together, we propose that the conserved Wdr8-hMsd1/SSX2IP complex plays a critical role in controlling proper spindle length and orientation.

Bipolar spindle

Centriolar satellites

Centriole

Centrosome

Fission yeast

Spindle pole body (SPB)

Super resolution microscopy

WD repeats

eng

Elsevier

© 2015 The Francis Crick Insitute. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

[ISSN] 0006-291X

[ISSN] 1090-2104

[DOI] 10.1016/j.bbrc.2015.10.169

[PMID] 26545777

[DOI] https://doi.org/10.1016/j.bbrc.2015.10.169