Difference in NaCl tolerance of membrane-bound 5′-nucleotidases purified from deep-sea and brackish water Shewanella species
Extremophiles 21 巻 2 号
357-368 頁
2017-01-03 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Difference in NaCl tolerance of membrane-bound 5′-nucleotidases purified from deep-sea and brackish water Shewanella species
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| 作成者 |
Kuribayashi Taka-aki
Masanari Misa
Yamanaka Masaru
Wakai Satoshi
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| 収録物名 |
Extremophiles
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| 巻 | 21 |
| 号 | 2 |
| 開始ページ | 357 |
| 終了ページ | 368 |
| 抄録 |
Shewanella species are widely distributed in sea, brackish, and fresh water areas, growing psychrophilically or mesophilically, and piezophilically or piezo-sensitively. Here, membrane-bound 5′-nucleotidases (NTases) from deep-sea Shewanella violacea and brackish water Shewanella amazonensis were examined from the aspect of NaCl tolerance in order to gain an insight into protein stability against salt. Both NTases were single polypeptides with molecular masses of ~59 kDa, as determined on mass spectroscopy. They similarly required 10 mM MgCl2 for their activities, and they exhibited the same pH dependency and substrate specificity for 5′-nucleotides. However, S. violacea 5′-nucleotidase (SVNTase) was active enough in the presence of 2.5 M NaCl, whereas S. amazonensis 5′-nucleotidase (SANTase) exhibited significantly reduced activity with the same concentration of the salt. Although SVNTase and SANTase exhibited high sequence identity (69.7%), differences in the ratio of acidic to basic amino acid residues and the number of potential salt bridges maybe being responsible for the difference in the protein stability against salt. 5′-Nucleotidases from these Shewanella species will provide useful information regarding NaCl tolerance, which may be fundamental for understanding bacterial adaptation to growth environments.
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| 著者キーワード |
Deep-sea
Growth environment
5′-Nucleotidase
NaCl tolerance
Shewanella
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| 内容記述 |
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan [No. 26240045], a grant from the Japan Society for the Promotion of Science [No. 25-1446], and The Salt Science Research Foundation [No. 1655].
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Springer Verlag
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| 発行日 | 2017-01-03 |
| 権利情報 |
© Springer Japan 2016
This is a post-peer-review, pre-copyedit version of an article published in Extremophiles. The final authenticated version is available online at: https://doi.org/10.1007/s00792-016-0909-8
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 1431-0651
[ISSN] 1433-4909
[DOI] 10.1007/s00792-016-0909-8
[PMID] 28050644
[DOI] https://doi.org/10.1007/s00792-016-0909-8
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