Intestinal absorption of the wheat allergen gliadin in rats

Allergology International 68 巻 2 号 247-253 頁 2019-04 発行
アクセス数 : 529
ダウンロード数 : 60

今月のアクセス数 : 1
今月のダウンロード数 : 0
ファイル情報(添付)
AI_68_247.pdf 731 KB 種類 : 全文
タイトル ( eng )
Intestinal absorption of the wheat allergen gliadin in rats
作成者
Fukushima Takahiro
Hamura Koh
Ninomiya Naoki
Ohashi Ryo
収録物名
Allergology International
68
2
開始ページ 247
終了ページ 253
抄録
Background: Aspirin enhances food allergy symptoms by increasing absorption of ingested allergens. The objective of this study is to elucidate the role of aspirin in facilitating intestinal absorption of the wheat allergen, gliadin, in rats.
Methods: Plasma concentrations of gliadin were determined after oral administration by gavage or administration into a closed intestinal loop in rats. We used an in situ intestinal re-circulating perfusion experiment to examine the effect of pepsin on aspirin-facilitated gliadin absorption. Fluorescein isothiocyanate (FITC)-labeled dextran-40 (FD-40) was used as a marker of non-specific absorption. The molecular size of gliadin and its allergenicity in plasma were examined using immunoblot analysis and intradermal reaction tests with Evans blue dye (EBD) extravasation, respectively.
Results: Aspirin increased plasma concentrations of gliadin after oral administration but had no effect in the closed intestinal loop study. An in situ intestinal re-circulating perfusion study showed that FITC-labeled gliadin was absorbed similarly to FD-40. Aspirin increased absorption of both intact and pepsin-digested gliadin, with a more significant effect on absorption of pepsin-treated gliadin. Immunoblotting showed that most gliadin was absorbed in intact form. When the gliadin fraction was extracted from rat plasma after gavage and injected intradermally into gliadin-sensitized rats, EBD extravasation was observed at injection sites in a gliadin dose-dependent manner.
Conclusions: Aspirin increased the absorption of intact and pepsin-digested gliadin via the paracellular pathway, maintaining their allergenicity. Moreover, the effect of aspirin on gliadin absorption was enhanced by modification and digestion of gliadin in the stomach.
著者キーワード
Absorption
Allergenicity
Aspirin
Gliadin
Pepsin
内容記述
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 16K08371).
NDC分類
医学 [ 490 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Japanese Society of Allergology
発行日 2019-04
権利情報
Copyright © 2018, Japanese Society of Allergology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 1440-1592
[DOI] 10.1016/j.alit.2018.11.005
[PMID] 30559050
[DOI] https://doi.org/10.1016/j.alit.2018.11.005