Determination of Fatty Acids in Human Sweat during Fasting Using GC/MS

Analytical Sciences Volume 26 Issue 8 Page 917-919 published_at 2010-08
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Title ( eng )
Determination of Fatty Acids in Human Sweat during Fasting Using GC/MS
Creator
Tsuda Takao
Kitagawa Kuniyuki
Source Title
Analytical Sciences
Volume 26
Issue 8
Start Page 917
End Page 919
Abstract
Fatty acids (FAs) are biological molecules that are used as major metabolic fuels, and are concerned in important metabolic processes. We have performed a non-invasive and technically rapid and simple method for collecting sweat from humans, followed by GC/MS determination. The sweat was collected from each volunteer (the middle finger) by spraying 70% ethanol aqueous solution (no harmful solvent) into a 1.5-cm3 plastic vial. Analysis of FAs in sweat showed that the sweat solution contains lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (C18:0). Here, it is demonstrated that FA concentrations for 4 young subjects correlated positively with percent of body fat (r = 0.78) and that the total FA levels for them increased progressively with increasing fasting time when a subject fasted throughout the experiment.
NDC
Chemistry [ 430 ]
Language
eng
Resource Type journal article
Publisher
The Japan Society for Analytical Chemistry
Date of Issued 2010-08
Rights
2010 © The Japan Society for Analytical Chemistry
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0910-6340
[ISSN] 1348-2246
[NCID] AA10500785
[DOI] 10.2116/analsci.26.917
[DOI] https://doi.org/10.2116/analsci.26.917