Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon
Microbiological Research 167 巻 5 号
311-316 頁
2012-05-20 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon
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| 作成者 |
Mizuta Mami
Satoh Emi
Katoh Chika
Tanaka Katsuyuki
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| 収録物名 |
Microbiological Research
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| 巻 | 167 |
| 号 | 5 |
| 開始ページ | 311 |
| 終了ページ | 316 |
| 抄録 |
Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation.
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| 著者キーワード |
Horizontal gene transfer
Conjugation
Transformation
Saccharomyces cerevisiae
Interaction between bacteria and eukarya
Gene transfer from bacteria to eukarya
Transkingdom sex
Broad host range plasmid
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| 内容記述 |
This work was supported in part by Japanese Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (#20570221).
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| NDC分類 |
生物科学・一般生物学 [ 460 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Elsevier GmbH
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| 発行日 | 2012-05-20 |
| 権利情報 |
© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0944-5013
[ISSN] 1618-0623
[DOI] 10.1016/j.micres.2011.10.001
[PMID] 22169356
[DOI] https://doi.org/10.1016/j.micres.2011.10.001
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