Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration

Takeda Katsuhiro

Sakai Noriyuki

Shiba Hideki

Nagahara Takayoshi

Fujita Tsuyoshi

Kajiya Mikihito

Iwata Tomoyuki

Matsuda Shinji

Kawahara Kazuko

Kawaguchi Hiroyuki

Kurihara Hidemi

Tissue engineering. Part A.

Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.

医学 [ 490 ]

英語

Mary Ann Liebert

This is a copy of an article published in the Tissue engineering, Part A. (c) 2011 Mary Ann Liebert, Inc.; Tissue engineering, Part A. is available online at: http://online.liebertpub.com.

[DOI] 10.1089/ten.TEA.2010.0070

[NCID] AA12306003

[ISSN] 1937-3341

[DOI] http://dx.doi.org/10.1089/ten.TEA.2010.0070 ～の異版である