Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization

Yanase Yuhki

Hiragun Takaaki

Uchida Kazue

Ishii Kaori

Oomizu Souichi

Suzuki Hidenori

Mihara Shoji

Iwamoto Kazumasa

Matsuo Hiroaki

Onishi Nobukazu

Kameyoshi Yoshikazu

Hide Michihiro

Biochemical and Biophysical Research Communications

We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production by B cells in vitro. In this study, we examined the effect of fucoidan on IgE production in vivo. The OVA-induced increase of plasma IgE was significantly suppressed when fucoidan was intraperitoneally, but not orally, administered prior to the first immunization with OVA. The production of IL-4 and IFN-gamma in response to OVA in spleen cells isolated from OVA-sensitized mice treated with fucoidan in vivo was lower than that from mice treated without fucoidan. Moreover, the flow cytometric analysis and ELISpot assay revealed that the administration of fucoidan Suppressed a number of IgE-expressing and IgE-secreting B cells, respectively. These results indicate that fucoidan inhibits the increase of plasma IgE through the suppression of IgE-producing B cell population, and the effect of fucoidan in VIVO is Crucially dependent on the route and timing of its administration.

Fucoidan

B cells

T cells

IgE

Ovalbumin (OVA)

医学 [ 490 ]

英語

Academic Press Inc

Elsevier Science

Copyright (c) 2009 Elsevier Inc.

[ISSN] 0006-291X

[DOI] 10.1016/j.bbrc.2009.07.031

[NCID] AA00564395

[DOI] http://dx.doi.org/10.1016/j.bbrc.2009.07.031