Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Journal of applied microbiology Volume 88 Issue 2 Page 191-201 published_at 2000
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Title ( eng )
Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture
Creator
Bainotti A. E.
Source Title
Journal of applied microbiology
Volume 88
Issue 2
Start Page 191
End Page 201
Abstract
The fermentative metabolism of Acetobacterium sp. grown on methanol–formate in continuous culture is described. The reaction stoichiometry of methanol-formate, including cells, were as follows: CH3OH + 1·13HCOOH → 0·87CH3COOH + 0·47 cell C. Formate enhanced growth yields by approximately 60% compared with methanol–CO2-grown cultures. Comparison of yields on methanol–formate allowed calculation of an energy yield of 1·3 mol ATP per mol acetate formed during homoacetate fermentation. The magnitudes of YEG,the theoretical maximum yield of YE, and m, the maintenance coefficient, were determined by growing the organism in methanol–formate and resulted in 16·5 g cell (mol methanol catabolized)-1 and 0·674 mmol methanol catabolized (g cell)-1 h-1, respectively. It is concluded that formate might replace CO2 as a source of carboxyl donor.
NDC
Biology [ 460 ]
Language
eng
Resource Type journal article
Publisher
Blackwell Publishing
Date of Issued 2000
Rights
Copyright (c) 2000 The Society for Applied Microbiology.
Publish Type Author’s Original
Access Rights open access
Source Identifier
The definitive version is available at www3.interscience.wiley.com
[ISSN] 1364-5072
[DOI] 10.1046/j.1365-2672.2000.00854.x
[NCID] AA11115564
[DOI] http://dx.doi.org/10.1046/j.1365-2672.2000.00854.x