A Possible Mechanism of Quinolone Resistance in Vibrio anguillarum.

Vibrio anguillarum のキノロン耐性機構

Okuda Jun

Kanamaru Shunsuke

Yuasa Akihiko

Nakaoka Noriyoshi

Kawakami Hidemasa

Nakai Toshihiro

魚病研究

Fish Pathology

Vibrio anguillarum のキノロン耐性機構を解明するために, DNAジャイレース遺伝子 (gyrA ) およびトポイソメラーゼIV遺伝子 (parC ) のキノロン耐性決定領域 (QRDR) 内の変異を解析した。低度耐性株ではgyrA のQRDRにのみ1塩基置換が検出された。一方, 高度耐性株ではgyrA とparC の両方のQRDRに1塩基置換が検出された。他菌種におけるキノロン耐性と同様, V. anguillarum においてもgyrA がまず変異することで低度耐性化し, 次にparC にも変異が入ることで高度耐性化すると考えられる。

To study a possible quinolone resistance mechanism in Vibrio anguillarum, we examined gyrA and parC mutations among the oxolinic acid-resistant strains and laboratory-derived mutants. One point mutation only in the quinolone resistance-determining region (QRDR) of the gyrA gene was detected in one strain isolated from ayu Plecoglossus altivelis and laboratory-derived mutants with low-level resistance. On the other hand, point mutations in both the gyrA and parC QRDRs were detected in one strain isolated from ayu and a laboratory-derived mutant with high-level resistance. These results suggest that as in other Gram-negative bacteria GyrA and ParC are the primary and secondary targets, respectively, of quinolone in V. anguillarum.

Vibrio anguillarum

quinolone resistance

gyrA

parC

oxolinic acid

Zoology [ 480 ]

eng

日本魚病学会

Copyright (c) 2007 The Japanese Society of Fish Pathology

[ISSN] 0388-788X

[ISSN] 1881-7335

[DOI] 10.3147/jsfp.41.73

[NCID] AN00063165

[DOI] http://dx.doi.org/10.3147/jsfp.41.73