Generation of hybrid hepatocytes by cell fusion from monkey embryoid body cells in the injured mouse liver
Histochemistry and Cell Biology 125 巻 3 号
247-257 頁
2006-03 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Generation of hybrid hepatocytes by cell fusion from monkey embryoid body cells in the injured mouse liver
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| 作成者 |
Okamura Kentaro
Asahina Kinji
Fujimori Hiroaki
Ozaki Rie
Shimizu-Saito Keiko
Tanaka Yujiro
Teramoto Kenichi
Arii Shigeki
Takase Kozo
Kataoka Miho
Soeno Yoshinori
Tateno Chie
Teraoka Hirofumi
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| 収録物名 |
Histochemistry and Cell Biology
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| 巻 | 125 |
| 号 | 3 |
| 開始ページ | 247 |
| 終了ページ | 257 |
| 抄録 |
Monkey embryonic stem (ES) cells have characteristics that are similar to human ES cells, and might be useful as a substitute model for preclinical research. When embryoid bodies (EBs) formed from monkey ES cells were cultured, expression of many hepatocyte-related genes including cytochrome P450 (Cyp) 3a and Cyp7a1 was observed. Hepatocytes were immunocytochemically observed using antibodies against albumin (ALB), cytokeratin-8/18, and α1-antitrypsin in the developing EBs. The in vitro differentiation potential of monkey ES cells into the hepatic lineage prompted us to examine the transplantabilityof monkey EB cells. As an initial approach to assess the repopulation potential, we transplanted EB cells into immunodeficient urokinase-type plasminogen activator transgenic mice that undergo liver failure. After transplantation, the hepatocyte colonies expressing monkey ALB were observed in the mouse liver. Fluorescence in-situ hybridization revealed that the repopulating hepatocytes arise from cell fusion between transplanted monkey EB cells and recipient mouse hepatocytes. In contrast, neither cell fusion nor repopulation of hepatocytes was observed in the recipient liver after undifferentiated ES cell transplantation. These results indicate that the differentiated cells in developing monkey EBs, but not contaminating ES cells, generate functional hepatocytes by cell fusion with recipient mouse hepatocytes, and repopulate injured mouse liver.
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| 著者キーワード |
cynomolgus
embryonic stem cells
hepatocyte differentiation
transgenic mouse
xenogeneic transplantation
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| NDC分類 |
生物科学・一般生物学 [ 460 ]
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Springer-Verlag
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| 発行日 | 2006-03 |
| 権利情報 |
Copyright (c) 2005 Springer-Verlag
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
The original version is abailable at www.springerlink.com
[NCID] AA11033302
[ISSN] 0948-6143
[DOI] 10.1007/s00418-005-0065-1
[PMID] 16195892
[DOI] http://dx.doi.org/10.1007/s00418-005-0065-1
～の異版である
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