The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants

Plant and Cell Physiology 48 巻 10 号 1484-1495 頁 2007-10 発行
アクセス数 : 838
ダウンロード数 : 170

今月のアクセス数 : 2
今月のダウンロード数 : 1
ファイル情報(添付)
PCP_48_1484.pdf 3.53 MB 種類 : 全文
タイトル ( eng )
The RNAi-mediated silencing of xanthine dehydrogenase impairs growth and fertility and accelerates leaf senescence in transgenic arabidopsis plants
作成者
Nakagawa Ayami
Sakamoto Saori
Morikawa Hiromichi
収録物名
Plant and Cell Physiology
48
10
開始ページ 1484
終了ページ 1495
抄録
Xanthine dehydrogenase (XDH) is a ubiquitous enzyme involved in purine metabolism which catalyzes the oxidation of hypoxanthine and xanthine to uric acid. Although the essential role of XDH is well documented in the nitrogen-fixing nodules of leguminous plants, the physiological importance of this enzyme remains uncertain in non-leguminous species such as Arabidopsis. To evaluate the impact of an XDH deficiency on whole-plant physiology and development in Arabidopsis, RNA interference (RNAi) was used to generate transgenic lines of this species in which AtXDH1 and AtXDH2, the two paralogous genes for XDH in this plant, were silenced simultaneously. The nearly complete reduction in the total XDH protein levels caused by this gene silencing resulted in the dramatic overaccumulation of xanthine and a retarded growth phenotype in which fruit development and seed fertility were also affected. A less severe silencing of XDH did not cause these growth abnormalities. The impaired growth phenotype was mimicked by treating wild-type plants with the XDH inhibitor allopurinol, and was reversed in the RNAi transgenic lines by exogenous supplementation of uric acid. Inactivation of XDH is also associated with precocious senescence in mature leaves displaying accelerated chlorophyll breakdown and by the early induction of senescence-related genes and enzyme markers. In contrast, the XDH protein levels increase with the aging of the wild-type leaves, supporting the physiological relevance of the function of this enzyme in leaf senescence. Our current results thus indicate that XDH functions in various aspects of plant growth and development.
著者キーワード
Arabidopsis (Arabidopsis thaliana)
plant growth and development
purine metabolism
senescence
xanthine dehydrogenase
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Oxford University Press
発行日 2007-10
権利情報
Copyright(c) 2007 Oxford University Press
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0032-0781
[DOI] 10.1093/pcp/pcm119
[NCID] AA0077511X
[DOI] http://dx.doi.org/10.1093/pcp/pcm119 ~の異版である