Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG(+) phenotype, lack of virulence and absence of a capsule
Letters in Applied Microbiology Volume 44 Issue 5
Page 481-487
published_at 2007-05
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| File | |
| Title ( eng ) |
Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG(+) phenotype, lack of virulence and absence of a capsule
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| Creator |
Kawanishi Michiko
Yoshida Teruyoyo
Kijima Mayumi
Yagyu Kazuhiro
Okada Sanae
Endo Akihito
Murakami Masaru
Morita Hidetoshi
Suzuki Shoko
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| Source Title |
Letters in Applied Microbiology
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| Volume | 44 |
| Issue | 5 |
| Start Page | 481 |
| End Page | 487 |
| Abstract |
Aims: To identify Lactococcus garvieae isolates from radish and broccoli sprouts and compare them with virulent and less virulent mutant strains obtained from yellowtails with regard to KG phenotype, presence of a capsule and virulence towards yellowtails and mice. Methods and Results: Comparative 16S rRNA gene sequence analysis of six isolates obtained from radish and broccoli sprouts indicated that they were L. garvieae (similarity >99%). They were compared with KG9502, Lg2 and ATCC49156 strains obtained from yellowtails. A less virulent mutant strain Lg2-S was obtained by Lg2 subculture. Biochemical characterization of the six strains resembled that of KG9502, Lg2, ATCC49156 and Lg2-S, except for saccharose and tagatose acidification and the presence of hippuricase. These six strains were nonpathogenic towards yellowtails and mice, nonsusceptible to bacteriophages and demonstrated heterogeneity on pulsed-field gel electrophoresis analysis. Using transmission electron microscopy, a capsule was observed in KG9502 and Lg2 but not in ATCC49156 and Lg2-S. Conclusions: We isolated L. garvieae strains that lacked pathogenicity towards yellowtails and mice from radish and broccoli sprouts; these were noncapsulated and exhibited KG+ phenotype. Significance and Impact of the Study: This is the first documentation of L. garvieae isolated from terrestrial plants. These isolates exhibited genetic diversity; however, they were noncapsulated and nonpathogenic towards yellowtails and mice.
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| Keywords |
Broccoli sprout
Japan
Lactococcus garvieae
Phage and pulsed-field gel electrophoresis
Phathogenicity
Radish sprouts
Seriola
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| NDC |
Industry and commerce [ 600 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Blackwell
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| Date of Issued | 2007-05 |
| Rights |
Copyright (c) 2007 Authors
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
The definitive version is available at www.blackwell-synergy.com
[ISSN] 0266-8254
[DOI] 10.1111/j.1472-765X.2007.02114.x
[NCID] AA10462245
[DOI] http://dx.doi.org/10.1111/j.1472-765X.2007.02114.x
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