Protein kinase Cδ binds TIRAP/Mal to participate in TLR signaling
Molecular Immunology Volume 44 Issue 9
Page 2257-2264
published_at 2007-03
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| File | |
| Title ( eng ) |
Protein kinase Cδ binds TIRAP/Mal to participate in TLR signaling
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| Creator |
Kubo-Murai Miho
Sukenobu Naoe
Yoshikawa Kyoko
Inoue Kazumi
Yamamoto Toshiyoshi
Matsumoto Misako
Seya Tsukasa
Inoue Norimitsu
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| Source Title |
Molecular Immunology
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| Volume | 44 |
| Issue | 9 |
| Start Page | 2257 |
| End Page | 2264 |
| Abstract |
Toll-like receptor (TLR) family members recognize specific molecular patterns within pathogens. Signaling through TLRs results in a proximal event that involves direct binding of adaptor proteins to the receptors. We observed that TIRAP/Mal, an adaptor protein for TLR2 and TLR4, binds protein kinase Cδ (PKCδ). TIRAP/Mal GST-fusion protein and a TIRAP/Mal antibody were able to precipitate PKCδ from rat peritoneal macrophage and THP1 cell lysates. Truncation mutants of TIRAP/Mal showed that the TIR domain of TIRAP/Mal is responsible for binding. TLR2- and TLR4-mediated phosphorylation of p38 MAPK, IKK, and IκB in RAW264.7 cells were abolished by depletion of PKCδ. These results suggest that PKCδ binding to TIRAP/Mal promotes TLR signaling events.
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| Keywords |
PKCδ
TLR
macrophage
signal transduction
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| NDC |
Medical sciences [ 490 ]
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| Language |
eng
|
| Resource Type | journal article |
| Publisher |
Pergamon-Elsevier Science Ltd.
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| Date of Issued | 2007-03 |
| Rights |
Copyright (c) 2006 Elsevier Ltd
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0161-5890
[DOI] 10.1016/j.molimm.2006.11.005
[PMID] 17161867
[NCID] AA00307483
[DOI] http://dx.doi.org/10.1016/j.molimm.2006.11.005
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