Effect of novel monoclonal antibodies on LIF-induced signaling in chicken blastodermal cells
Developmental and Comparative Immunology 30 巻 5 号
513-522 頁
2006 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Effect of novel monoclonal antibodies on LIF-induced signaling in chicken blastodermal cells
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| 作成者 |
Yamashita Yusuke
Tategaki Airo
Ogawa Mari
Nishida Kensho
Akita Sachiko
Matsuda Haruo
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| 収録物名 |
Developmental and Comparative Immunology
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| 巻 | 30 |
| 号 | 5 |
| 開始ページ | 513 |
| 終了ページ | 522 |
| 抄録 |
Leukemia-inhibitory factor (LIF) is indispensable for maintaining the undifferentiated state when propagating mouse embryonic stem (ES) cells. We previously cloned chicken LIF (chLIF) cDNA and demonstrated that it maintained chicken ES cell cultures in an undifferentiated state. Here, we developed two monoclonal antibodies, HUL-1 and HUL-2, against chLIF, which specifically recognized recombinant chLIF (rchLIF) produced by Escherichia coli and Chinese hamster ovary K1 cells, in enzyme-linked immunosorbent assays and Western blot analysis. In addition, HUL-2 inhibited the phosphorylation of signal transducer and activator of transcription 3 by rchLIF in chicken blastodermal cells (CBCs), but not that of mitogen-activated protein kinase kinase. Furthermore, the addition of HUL-2 to CBC cultures resulted in embryoid bodies forming earlier than in normal cultures. These results indicated that HUL-2 recognized not only rchLIF but also native chLIF, and suggested that CBCs in culture produce LIF, which functions in autocrine signaling.
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| 著者キーワード |
chicken
chicken blastodermal cells
cytokine
ES cells
LIF
mAb
MEK1/2
STAT3
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| NDC分類 |
生物科学・一般生物学 [ 460 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Elsevier
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| 発行日 | 2006 |
| 権利情報 |
Copyright (c) 2006 Elsevier Ltd.
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0145-305X
[DOI] 10.1016/j.dci.2005.07.003
[NCID] AA00627870
[PMID] 16150488
[DOI] http://dx.doi.org/10.1016/j.dci.2005.07.003
～の異版である
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