Synergistic defect in 60S ribosomal subunit assembly
Nucleic acids research Volume 33 Issue 14
Page 4553-4562
published_at 2005
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| File | |
| Title ( eng ) |
Synergistic defect in 60S ribosomal subunit assembly
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| Creator |
Nariai Masanobu
Tanaka Tomohisa
Okada Takafumi
Shirai Chiharu
Horigome Chihiro
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| Source Title |
Nucleic acids research
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| Volume | 33 |
| Issue | 14 |
| Start Page | 4553 |
| End Page | 4562 |
| Abstract |
Rrs1p, a ribosomal protein L11-binding protein, has an essential role in biogenesis of 60S ribosomal subunits.Weobtained conditionallysynthetic lethal allele with the rrs1-5 mutation and determined that the mutation is in REX1, which encodes anexonuclease. The highly conserved leucine at 305 was substituted with tryptophan in rex1-1. The rex1-1 allele resulted in 30-extended 5S rRNA. Polysome analysis revealed that rex1-1 and rrs1-5 caused a synergistic defect in the assembly of 60S ribosomal subunits. In vivo and in vitro binding assays indicate that Rrs1p interacts with the ribosomal protein L5.5S rRNA complex. The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5.5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p.
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Oxford University Press
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| Date of Issued | 2005 |
| Rights |
Copyright (c) 2005 Oxford University Press
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| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0305-1048
[DOI] 10.1093/nar/gki772
[DOI] http://dx.doi.org/10.1093/nar/gki772
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