Synergistic defect in 60S ribosomal subunit assembly
Nucleic acids research 33 巻 14 号
4553-4562 頁
2005 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Synergistic defect in 60S ribosomal subunit assembly
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| 作成者 |
Nariai Masanobu
Tanaka Tomohisa
Okada Takafumi
Shirai Chiharu
Horigome Chihiro
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| 収録物名 |
Nucleic acids research
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| 巻 | 33 |
| 号 | 14 |
| 開始ページ | 4553 |
| 終了ページ | 4562 |
| 抄録 |
Rrs1p, a ribosomal protein L11-binding protein, has an essential role in biogenesis of 60S ribosomal subunits.Weobtained conditionallysynthetic lethal allele with the rrs1-5 mutation and determined that the mutation is in REX1, which encodes anexonuclease. The highly conserved leucine at 305 was substituted with tryptophan in rex1-1. The rex1-1 allele resulted in 30-extended 5S rRNA. Polysome analysis revealed that rex1-1 and rrs1-5 caused a synergistic defect in the assembly of 60S ribosomal subunits. In vivo and in vitro binding assays indicate that Rrs1p interacts with the ribosomal protein L5.5S rRNA complex. The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5.5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p.
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Oxford University Press
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| 発行日 | 2005 |
| 権利情報 |
Copyright (c) 2005 Oxford University Press
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0305-1048
[DOI] 10.1093/nar/gki772
[DOI] http://dx.doi.org/10.1093/nar/gki772
～の異版である
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